Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 11th Global Gastroenterologists Meeting Hotel Holiday Inn Rome Aurelia Via Aurelia Km 8400, Rome, Italy.

Day 1 :

Keynote Forum

Francesco Marotta

ReGenera Research Group for Aging Intervention, Italy & MMC Clinic - Gender Medicine Unit by Genomics & Biotechnology, Milano, Italy

Keynote: Hormetic Microbiota effect on the gut-based mechanism of Metformin benefit

Time : 09:00-09:40

Conference Series Gastro 2017 International Conference Keynote Speaker Francesco Marotta photo
Biography:

Francesco Marotta MD, Ph.D. with experience in gastroenterology, oxidative stress, aging and nutrigenomics in the USA, Cape-Town, and Japan leading to extensive publications. Hon. Research Professor at Dept of Nutrition, Texas Women University, USA, Advisory Board Panel of the Center for Life Science at Nazarbayev University, Astana, Kazakhstan and External Examiner at McGill and Osaka Universities ( Canada and Japan). Currently, he serves as CMO at Milano Medical center for Healthy Aging and also at a prime international clinical set up in Central-Asia,

Abstract:

Metformin is commonly used as the first line of medication for the treatment of metabolic syndromes, such as obesity and type 2 diabetes (T2D). Recently this compound has gained an increasing interest within the scenario of pro-longevity medicine given also its peculiar increase in AMP-activated protein kinase (AMPK) thus beneficially affecting  energy balance by help maintaining a proper cellular AMP/ATP ratio through the increase of ATP consumption and decreasing ATP production, which is associated with AMPK activation.
The gut microbiota is known to play an important role in harvesting energy from food, metabolic processes, and immune modulation. An increasingly body of evidence proves that its composition is significantly associated with obesity, T2D, metabolic syndromes and other chronic diseases. 
Data show that when metformin is administered to high fat diet (HFD) animals, the composition of the phylum Bacteroidetes significantly increases (over 75%)  similar to that in the normal diet-(ND)-fed animals. Moreover, the composition of Verrucomicrobia in the HFDMet group significantly increases, unlike what obtained by simple dietary change applied to HFD. In the HFD-Met group, the abundances of the families Bacteroidaceae, Verrucomicrobiaceae, and some specific Clostridia change significantly vs those in the HFD and HFD-ND groups. Interestingly, Metformin treatment also affects the composition of the gut microbiota in mice fed a ND. The families Rikenellaceae, Ruminococcaceae, and Verrucomicrobiaceae, as well as Alistipes spp., Akkermansia spp., and Clostridium spp., are more abundant in the ND-Met group than the ND group. All this is starting shedding new light on the AMPK-independent pathway of metabolic improvement by metformin treatment through targeting the microbiota. It is likely that metformin via changes in Akkermansia and Lactobacilli bacteria regulation  improves the metabolic profile of diet-induced obesity by ameliorating low-grade tissue inflammation and also upregulating the intestinal expression of several endocannabinoids controlling inflammation, barrier function and peptide secretion in the gut.
Till recently, the main hindrance in bacterial stool culture is represented by the major bias that only a few gut bacteria can be properly detected and cultivated in the laboratory. On the other hand, capillary sequencing or PCR-based approaches need culture medium with its inner complexities of multiple separate analysis. In this scenario, a rising star is represented by the next-generation sequencing (NGS) which, by combining multiple samples in a sequencing run, is able to analyse the entire microbial community within a sample. Thus this unique ability enables  to catalog resident organisms within the very complex gut polymicrobial bacterial communities to make a DNA-stable sampling and producing a report intelligible to physicians and ideally endowed with a nutritionist and a gastroenterologist commented interplay so to make it a valid diagnostic, a treatment-guided result and a follow-up tool as well. This has been quite recently achieved by a spin-off of dedicated geneticists and biologists (Next Genomics, Prato, Italy)  using a kit allowing small and 14-day stavle sampling of  0,0001% accuracy. This test is currently used also in the clinical center (MMC Milano, Milan, Italy) helping to identify the enterotype and the presence of bacterial species correlated with diseases.
 

 

Keynote Forum

Larry I Good

Good Pharmaceutical Development Company, USA

Keynote: Serum derived bovine immunoglobulin in the treatment of gastrointestinal disease

Time : 09:40-10:20

Conference Series Gastro 2017 International Conference Keynote Speaker Larry I Good photo
Biography:

Dr. Larry Good has been a practicing gastroenterologist since 1978. Dr. Good graduated Colgate University Magna Cum Laude in 1969 and received his M.D. with Alpha Omega Alpha status from the Medical University of South Carolina in Charleston, SC in 1973.

Dr. Good served a medical residency from 1973-76 and was Chief Medical Resident in 1976. He was fellowship trained in gastroenterology from 1976-78.

Dr. Good has served as the Director of Liver Diseases at Nassau County Medical Center and was for many years Chief, Division of Gastroenterology, Department of Medicine at South Nassau Communities Hospital. Dr. Good is an assistant clinical professor of medicine at SUNY Stony Brook. He has given hundreds of lectures in his field and has authored numerous papers and abstracts. Recently, Dr. Good presented the ACG Theater lecture at the American College of Gastroenterology annual meeting in Chicago, Illinois in October, 2014.

Dr. Good was Chief Medical Officer at Ritter Pharmaceuticals in Los Angeles, California, where he expanded his research interest in the intestinal microbiome. Dr. Good’s current clinical research activities involve the microbiome, inflammatory bowel disease, irritable bowel syndrome and the application of orally administered gamma globulin to patients with acute and chronic gastro-intestinal disorders.

Abstract:

Serum derived bovine immunoglobulin was introduced as a medical food in the U.S. in 2013. Since then it has been studied in over 800 patients with a variety of gastrointestinal illnesses including diarrhea predominant irritable bowel syndrome (IBS-D), ulcerative colitis, Crohn’s disease, pouchitis, C. difficile colitis and chronic mesenteric ischemia. Its effectiveness in these diverse disease entities is related to the ability of SBI to bind intraluminal pro-inflammatory mediators including enterotoxins, pro-inflammatory cytokines and bacterial degradation products, thereby, preventing the loss of intercellular tight junction proteins. By maintaining tight junction, integrity, dendritic antigenic stimulation in the mucosa is reduced, resulting in inhibition of the inflammatory cascade.           This presentation will review the US FDA category of medical foods, the proposed mechanism of action SBI and published clinical data.

  • The Gastroesophageal Reflux Disease: a focus on the situation and therapeutic`advances.
Location: Olimpica 2
Speaker

Chair

David H. Van Thiel

Advanced Liver and Gastrointestinal Disease Center, USA

Speaker

Co-Chair

Antonio Iannetti

University of Rome “La Sapienza, Italy

Session Introduction

David H Van Thiel

Advanced Liver and Gastrointestinal Disease Center, USA

Title: GERD: Recognition, Diagnosis, and Treatment as seen by a non-esophagologist

Time : 10:40-11:00

Speaker
Biography:

David Van Thiel is a gastroenterologist in Berwyn, Illinois. He is affiliated with multiple hospitals in the area, including Rush Oak Park Hospital and Rush University Medical Center. He received his medical degree from David Geffen School of Medicine at UCLA and has been in practice for 39 years. Dr. Van Thiel accepts several types of health insurance, listed below. He is one of 21 doctors at Rush Oak Park Hospital and one of 25 at Rush University Medical Center who specialize in Gastroenterology. Dr. Van Thiel graduated from the University of California at Los Angeles. Dr. David H Van Thiel, MD has obtained board certification from the member board for Internal Medicine and Hepatology.
 

Abstract:

Gastroesophageal reflux is a condition that occurs when gastric (GERD) or gastroduodenal (EDRD) content refluxes into the esophagus.  Depending upon the degree to which duodenal secretions contaminate the gastric refluxate the refluxed material can be acidic (pH less than 4 for gastric reflux), mildly acidic (pH ranging between 4 but < 7, mildly ascitic), or alkaline (pH greater or equal 7).  The patient's symptoms are a reflection of the degree of tissue inflammation plus or minus tissue injury (erosions or ulceration) produced rather than the specific nature of the refluxed material.  Continued uncontroled/chronic reflux leads to the complications of GERD/GDRD consisting of inflammation that can progress to erosions, ulcerations, or scarring (stricture), mucosal metaplasia (Barrett's esophagus) or adenocarcinoma.

GERD is a common disorder with a world prevalence that somewhat between 10 and 30% of the population being less commonly seen in Asia as compared to Europe or the Americas.  Clinical GERD is estimated to be seen in 7% of the population is  manifested as  ‘ heartburn" and accounts for 4% of the visits to primary care physicians and is the most common reason for referral to gastroenterologist.

Complicated GERD is manifested by “alarm signals” consisting of weight loss, odynophagia, dysphagia, regurgitation, anemia and a variety of extra- esophageal symptoms ascribed to GERD consisting of hoarseness, laryngitis, bronchitis, dental enamel erosion, otitis media, sinusitis, pseudo- angina, sleep dysfunction, asthma, pneumonitis, and pulmonary fibrosis.  In addition, as a consequence of the almost universal use of proton pump inhibitors for the treatment of GERD an increase in cases of community-acquired and hospital-acquired pneumonia have been reported in the elderly as a direct consequence of the reduction in the acid secretion by the stomach and the resultant increase in viable gastric microbial concentrations.

Risk factors for GERD are common in the population and consist of the existence of a hiatal hernia, obesity, type 2 diabetes mellitus, hypertension, hyperlipidemia, advanced age, chronic hiccups, asthma, and less often sinusitis, chronic recurrent otitis media and dental erosions.  Because heartburn can be treated very effectively with proton pump inhibitors there is no need for endoscopy or other invasive diagnostic procedures to establish a diagnosis in cases of simple heartburn, GERD, or GERD.  The use of endoscopy, pH monitoring, with without impedance determination, and esophageal manometry are reserved for those individuals manifesting "alarm symptoms", those who have failed treatment with a proton pump inhibitor and those being considered for reflux surgery.  Endoscopy is used to grade the severity of reflux inflammation and the identification of erosions or ulcerative disease and assess the patient for the more severe or advanced complications consisting of stricturing, Barrett's esophagus and/or adenocarcinoma.  Ambulatory pH monitoring is used to document reflux and define its character (acid, mildly acid, or alkaline), its frequency, and duration as well as its relationship with symptoms as reported by the patient.  Manometry enables a quantitative assessment of esophageal motility and measurement of the frequency of transient esophageal relaxations, and the duration of the reflux exposure as well as the ability to rule out the presence of achalasia, a contraindication to reflux surgery.

Reproducible therapies of GERD consist of the administration of proton pump inhibitor taken one to 2 hours before the first meal of the day for 12 weeks.  Failure to respond to find a symptomatic relief is managed by a doubling of the morning  proton pump inhibitor dose with or without the addition of an H2 receptor antagonist at that time which is utilized particularly for those with nocturnal reflux symptoms or sleep apnea.

Prokinetic agents are used exclusively when alkaline reflux is present to prevent alkaline reflux by increasing esophageal contractions (esophageal clearance), to increase lower esophageal resting pressure to prevent reflux ,  and enhancement of gastric and duodenal motility there by reducing the volume of gastric/duodenal content available for reflux.  The use of these agents is limited by the development of tolerance within a short period of time, their short duration of action, and their adverse effects consisting of tremor, fatigue and cardiac arrhythmias. 

A large number of abnormal anatomic variations at the gastroesophageal junction and physiologic and neuroendocrine reflexes occur  as high frequency in cases of GERD and our thought  to contribute to the pathophysiology of the disorder.  Disease consist in part consist of lower esophageal sphincter incompetence, reduced resting lower esophageal pressure, the presence of a hiatal hernia, reduced esophageal clearance, increased frequency of transient esophageal relaxations, esophageal, gastric and/or duodenal motility disorders.  With the exception of surgical repair of anatomic variants, the identification of these abnormalities has not contributed substantially to the treatment of GERD/GDRD.  This is particularly true of the research directed at the identification of neuro- and endocrine mechanisms that could contribute to esophageal dysfunction and reflux.

For those who fail extended proton pump therapy or develop complications including an esophageal stricture, Barrett's esophagus, esophageal cancer, chronic intractable reflux symptoms often manifested as chronic hiccups, or morbid obesity, surgery is the treatment of choice and consists of any variation of the Nyssen procedure done laparoscopically or any of the variations bariatric surgery.  These surgical procedures produce long-term benefit but often with minor relapses that necessitate the use of an additional acid inhibitory agent for a PPI or H2 receptor antagonists and occasionally a prokinetic agent as well.

Recently, a large variety of endoscopic procedures consisting suturing the lower esophageal sphincter, Silastic implants and endoscopic fundoplication have been developed with for use in the treatment of GERD, but these agents are agents are still investigational and have not yet obtained FDA approved

Giuseppe Del Buono

Clinica Pio XI Roma, Italy

Title: Diagnosis: when the pH-impedance and manometry?

Time : 11:00-11:20

Speaker
Biography:

He graduated in medicine and surgery, on 11/12/1981. The same year he entered graduate school in Digestive System Diseases directed by Prof.Aldo Torsoli, working in particular with his young researcher, now a professor of gastroenterology in Rome: prof. Enrico Corazziari. Since then I have always been interested in the study of the pathophysiology of the Digestive Tract and neurogastroenterology. Up to now, he focus my studies on organic and functional disorders of the digestive system. For years he work with the best specialists in every sector and structure, public and private, to understand and solve a 360 ° diagnostic and therapeutic issues of Gastroenterology.

He have to my credit numerous publications and participation at conferences. He maintain, the beginning of specialization, the study of the motility of the Digestive Tract.

He always run Digestive standard manometry, pH-metry, pH-Impedance, CH4 H2-Breath Test, Pelvic Floor Rehabilitation (pelviperineale pain urogineproctologico origin, urinary incontinence, obstructed defecation syndrome, fecal incontinence, etc.) its rehabilitation treatment.

Currently, in October 2014, my army activities at the Clinica Pio XI, Via Aurelia 559 Rome, with new and sophisticated equipment (manometry HIGH RESOLUTION with a neurological module for EMG).

Abstract:

The Esophageal manometry is a diagnostic test used in gastroenterology to study esophageal motility.
 
The examination is performed by placing a naso-gastric tube into the esophagus of the patient.
With esophageal manometry it is therefore possible to follow the peristaltic wave of the esophagus during swallowing and to verify any motor esophagus (dysphagia abnormalities, achalasia, diffuse esophageal spasm).
 
The High Resolution Esophageal manometry, using the latest generation sensors, provides the opportunity to give, at the same time, immediate information of the entire upper digestive tract.
The new technology, with the latest "Classification of Chicago", provides a precision and ease than ever before diagnosis. The examination of esophageal manometry High Resolution is done through the introduction nasally trans of a catheter with variable number of sensors from 24 to 36 depending on the technology. This allows the complete study of deglutitiva phase maintaining the tube in one location, with the result to obtain:
 
Full results and coordinated activities of the entire deglutitiva.
Faster execution of the examination.
Increased compliance examination by the patient
 
The Ph-metry esophageal 24 hours allows to measure the pH of the esophageal tract for 24 hours, and then determine the number of reflux of acid type had. This examination is carried out by inserting one nostril through a naso-gastric tube and placing it into the esophagus. The tube is connected to an instrument, very light and compact, which will record the pH for the entire 24 hours. After placing the tube of pH monitoring, the patient will go home, trying to spend the day in a manner more similar to normal.
 
The examination of pH-metry has some limits: it indicates, in fact, only episodes of reflux of acid type, ie if the pH has fallen below the value of 4. Also it can not determine exactly how high it gets into the esophagus reflux. Symptomatic patients may have a perfectly normal manometry and pH-metry negative!
 
These problems have been overcome by pH-impedance of 24 hours. It is used a naso-gastric tube on which is positioned a pH sensor (2 times) and 6 channels which measure the electrical impedance.
The impedance (is an electrical resistance) is measured by the instrument and its value varies with the bolus passage. Having six impedance channels on the tube you can not discriminate against a swallowing by a reflux and also know how high it's reflux into the esophagus and duration of reflux itself. In addition, since the tube is present a pH sensor, it is also possible to know the degree of acidity of the reflux. The combined pH and impedance measurements have made it possible to classify new categories of reflux:
 
Refluxes acids (pH <4 such as the pH-metry classic examination).
Refluxes repeated acids while the pH is <4, normally recognized by pH-metry as a single episode of reflux.
Refluxes mildly acidic with pH> 4 but <7.
Refluxes not with acid pH> 7.
 

Francesco Falbo, Paolo Urciuoli

University of Rome "La Sapienza",

Title: Surgical therapy: a review and technological innovations

Time : 11:20-11:40

Speaker
Biography:

 
Paolo Urciuoli, born in Turin on July 28, 1959, received his classical studies in 1978 with honors in the same year he enrolled in the Faculty of Medicine, University of Rome "La Sapienza", where he graduated 25 July 1984 with 110/110 cum laude. In November 1984 he passed the state examination for the qualification to the profession of Surgeon Doctor at the University of Rome "La Sapienza" with subsequent registration in the of the Medical Association of Rome and Province (No. 36022). In 1989 he specialized in general surgery with 70/70 and honors.  In 2000 he specialized in vascular surgery with 70/70 and honors  over the years 1990/1991 she completed an internship at Thomas Jefferson University in Philadelphia, USA in surgery general and colo-proctology. Since January 2, 1990 is a Researcher at the Institute of Surgical Clinic III (currently Department of Surgical Sciences). Since 2003/2004 got the teaching position in surgical oncology Internal Medicine and General Surgery I, V year, the Bachelor of Medicine B, The Faculty, University "La Sapienza"
 

Abstract:

Introduction. American Gastroenterologists Association 2008 guidelines suggests antireflux surgery to be reserved for patients with esophagitis and intolerance to PPI and patients with poor control of the gastro-esophageal reflux symptoms, especially regurgitation. Laparoscopic Nissen-Rossetti fundoplication has become the gold standard, being a well tolerated operations and considering its good outcome in terms of symptoms relief.

Laparoscopic Nissen-Rossetti fundoplication. In 1939 Rudolph Nissen improvised a fundoplication to protect an esophagogastric anastomosis. Some years later he performed this procedure to treat gastro-esophageal reflux disease and published the first description of the procedure in 1956. What we now call Nissen-Rossetti fundoplication is the result of the contribution by Nissen’s favourite pupil, Marco Rossetti. Nissen-Rossetti fundoplication consists in an extensive mobilization of the posterior wall of the stomach, which enables a loose wrap of the anterior wall to be used for the total wrap, without the division of the short gastric vessels. This procedure showed good results in term of post-operative dysphagia. With the extensive application of laparoscopic surgery during the 1990s, the volume of antireflux surgery increased. At the present time Nissen-Rossetti compare favorably in terms of mortality and morbidity with appendectomy and cholecystectomy.

Technological innovations. Where is antireflux surgery going from now on? First of all, robotic-assisted surgery. Although as of now robot-assisted surgery still has an unacceptable high cost for benign pathology, numerous studies are reporting comparable results in terms of outcomes versus laparoscopic surgery.

An interesting new device is LINX©, or Magnetic Sphincter Augmentation (MSA), a small flexible band of interlinked titanium beads with magnetic cores that works by restoring the continence of the lower esophageal sphincter. This device can be easily placed around the gastro-oesophageal junction in about 30 minutes.However it needs longer follow-up and has some limitation: it can’t be used in hiatal hernias larger than 3 cm, the safety and effectiveness of the LINX device has not been evaluated in patients with Barrett's esophagus or Grade C or D (LA classification) esophagitis and in patients with electrical implants such as pacemakers and defibrillators, or other metallic, abdominal implants.

Conclusions. LNR procedure should be considered the gold standard to treat patient with refractory GERD. 2013 UK REFLUX trial concluded that a surgical policy is probably cost-effective, considering LNR in a 5 years follow-up provided a better health-related quality of life compared with medical management. Waiting for a mininvasive techniques standardisation and long term follow up, patients should be aware of the safeness and feasibility of laparoscopic Nissen-Rossetti fundoplication. 

Speaker
Biography:

Antonio Iannetti has done his degree in Medicine and Surgery and Specialties in "Gastroenterology" and "Internal Medicine" at the University of Rome. 1980-1983 University of Los Angeles (USA), he is interested endoscopic sclerosis of esophageal varices and retrograde cholangiopancreatography-endoscopically. He is University Professor and Chair of Gastroenterology - University of Rome. He is head of the Digestive Endoscopy Service of the University Hospital Umberto I in Rome. He is an expert of the Ministry of Health for Gastroenterology. 

Abstract:

Introduction: The incidence of gastroesophageal reflux disease in the population of industrialized countries is high and ranges from 20 to 40% in the age groups between 45-64 years, with a further increase in the incidence in the age between 64-74 years. The natural history of the disease requires continuous recrudescence alternated with quiescent phases. In view of these epidemiological data, the importance of the social problem and the high health costs is cleared. It follows the interest of pharmaceutical companies, the companies of electromedical and producing toolkits endoscopic and surgical companies.

Objective: In this session, I intend, with the participation of colleagues internists and surgeons, to make a brief stock of the situation, about the gastro-esophageal reflux disease. I will make a tour of the clinical presentation, the increase of incidence, especially of so-called atypical forms and symptoms of gastro-pharingeal reflux (high reflux), emphasizing how many patients are refractory to therapy. Patients who benefit from medical treatment, they become dependent on care. Whereas, many are young and that medical therapy has adverse side effects, such as anemia, osteoporosis, and infections, is the need for alternative therapies. Physiotherapy global posture, for example, can be a transient and partial support. The ultimate solution is or should be surgical.

Considerations: Surgical therapy makes use of minimally invasive or laparoscopic method, which shortens the hospital stay. But an endoscopic surgery, easy, repeatable, free from postoperative complications, can be performed in day surgery, would be ideal for this type of chronic disease. In reviewing the different techniques, that have been proposed over the last 20 years, I relate the considerations, derived from the international literature. This presentation is concluded by presenting a last device, manufactured in Germany, derived from its precursor, the NDO Plicator, which is making use of the addition of heads polytetrafluoroethylene (PTFE), which retain the suture threads from the traction, exerted by the tissues, seem to improve the seal in time.

Conclusions: I carry scientific studies that have compared the operations, performed with GERD-X Plicator, to surgical interventions of fundoplication, with satisfactory results. My invitation is to continue to seek solutions with endoscopic surgery, which is the most appropriate technique for this type of pathology.

Speaker
Biography:

Univ. Prof. Dr. Rudolph Pointner is current working in Tauernklinikum Zell am See under Department of Allgemein- und Visceralchirurgie. 
 

Abstract:

The GERD-X procedure is a further development of the NDO-Plicator-System consisting of a flexible endoscope-like tube and a playstation-like operating console. In the front of the tube the needles and pretied sutures are hold in a closed snake- or crocodile-like mouth. The procedure is performed under direct vision of a retroflexed babyscope, which is passed through the inserted tube in a patient under general anaesthesia in normal back position. Once in appropriate position at the left side of the GE-junction the arms of the device are closed, deploying the implant which is made of pretied sutures and PTFE-blades. Subsequently after realising this first step, the device is removed, leaving a full thickness permanent suture plication of the gastric wall. This procedure is reproducible until the GE-junction is tight and shows a fundoplication-like gastroesophageal valve Hill Grade I. 
 
Goal of the presentation is to present a series of 120 patients assigned to the GERD-X procedure. In all those patients a meticulous preoperative examination was performed, including endoscopic examination, cinematographic esophageal barium X-Ray-Studies, High resolution Manometry, Impedance pH-monitoring and quality of life score studies using the Gastrointestinal Quality of Life-Score according to Eypasch and Troidl. Exclusion criteria for performing the GERD-X-procedure in this series were defined as Hiatal hernias more than one centimetre detected in high resolution manometry and/or a gastroesophageal valve more than Hill Grade II.
 
Considerations: According to a detailed follow-up and analysis of our patients the focus for achieving good results has to be put on the exclusion criteria of a Hiatal hernia or gastroesophageal valve more than Hill Grade II and a sophisticated handling of the GERD-X device. Meeting these presumptions excellent results corresponding to the GIQLI-score can be achieved, although the number of reflux episods decreases only to the middle compared to a decrease of about 90% after a Nissen procedure. 
 
Conclusion: The special merits of the GERD-X procedure are a high rate of patient – satisfaction according to the GIQLI – scores without producing complications or side effects as they are known after laparoscopic fundoplication.
 

  • NEW THERAPEUTIC POSSIBILITIES IN DIGESTIVE ENDOSCOPY WITH PARTICULAR REFERENCE TO THE PROBLEM OF OBESITY AND METABOLIC SYNDROME: AN INTERNATIONAL REVIEW
Location: Olimpica 2
Speaker

Chair

A. Iannetti

University of Rome “La Sapienza, Italy

Co-Chair

Prof. Andrea Formiga,

Istituti Clinici Zucchi Monza (Italy)

Session Introduction

Alfredo Genco

Sapienza University, Italy

Title: Endoscopic therapy of obesity
Biography:

Abstract:

Andrea Formiga

Istituti Clinici Zucchi Monza (Italy) – Gruppo San Donato

Title: Abstract Endobarrier System, Duodenal Jejunal Endoscopic linear bypass

Time : 13:40-14:00

Speaker
Biography:

Dr. Andrea Formiga has completed his PhD at the age of 25 years in Milan University Italy. In  2002 he has completed the postdoctoral speciality in general surgery. He is the director of the General Surgery Department of Istituti Clinici Zucchi of Monza (Italy) – Gruppo San Donato.

Member of International Advisory Board for Apollo Endosurgery

He has published many documents in surgical and endoscopic treatments for general surgery and obesity procedures.

He has partecipate to many national and international congresses.

Abstract:

The Endobarrier system is an innovative reversible endoscopic system indicated for the treatment of diabetes mellitus type II and obesity. The duration of treatment is one year.

The process involves a decrease in glycated hemoglobin of 2 points in the year of treatment with reduction and/or elimination of oral hypoglycemic agents and/or units of insulin administered daily, improved lipid profile and liver function tests, reduction of blood pressure, and weight loss of about 40% of excess body weight. The system is totally endoscopic, simulates the effect obtained with a laparoscopic gastric bypass but without making sections and/or removal organ.

Method: From March 2014 to November 2016 we performed 20 placements of Endobarrier system (15 men, 5 women), of whom 16 successfully concluded and extracts, 4 still in progress; 2 System extractions of foreign patients who had undergone placement in other countries (Australia, South Arabia), 1 missed positioning for non-compliant patient anatomy (inability to access the capsule in the duodenal bulb). The average hospital stay of patients was 1 day. The mean age of patients was 46 (24-63 aa) range. All positioning and extraction operations were performed under general anesthesia.

Results: The mean preoperative BMI was 45.8, average BMI 37.8 postoperatively; preoperative Hb A1c 8.9 / postoperative HbA1c 6.6; PA values systolic / diastolic mean preoperative 134 / 85.7, PA values postoperative systolic / diastolic 124.7 / 71.8.

Complications: we found the appearance of a duodenal ulcer bleeding in the vicinity of the metal anchoring system of the system that was treated with medical drugs and with the removal of the system; in the same patient appearance of mild pancreatitis with high levels of amylase (400) and lipase (200) resolved spontaneously after removal.

One hepatic abscess in a foreign patient came to our attention from other structure to which it was addressed in urgency.

Conclusions:

The endoscopic endoluminal treatments are now having great expansion and interest even in the treatment of morbid obesity and diabetes

The Endobarrier System is an innovative totally endoscopic malabsorptive reversible treatment that can have an important role in the treatment of diabetes mellitus type II and obesity.

Our experience has shown the effectiveness and feasibility of Endobarrier system. It remains to evaluate the results in the time after removal.

Speaker
Biography:

Kenji Sasaki completed his MD and, as an Immunologist, he completed his PhD at Tohoku University School of Medicine. He was trained at Miyagi Cancer Center. He is a Board Certified Fellow and Preceptor of Japan Gastroenterological Endoscopy Society, Board Certified Gastroenterologist of Japanese Society of Gastroenterology, Board Certified Member of the Japanese Society of Internal Medicine and Editorial Board Member of CRIM. He has published several papers on Gastroenterology in international journals and served as a Reviewer for Journal of Medical Microbiology, Journal of Pharmacology & Pharmacotherapeutics and Journal of Gastrointestinal & Digestive System.

Abstract:

A 66-year-old Japanese male was shown to have severe ulcers with hypergastrinemia in the stomach through proximal horizontal part of the duodenum. He suffered from gastric ulcer at 63 and hyperparathyroidism at his 5th decade. Though he had no definitely enlarged pituitary detectable by computed tomography, he had slight defects in the visual field and hyperprolactinemia. A diagnosis of multiple endocrine neoplasia type 1 (MEN1) was entertained. Follow up EGD revealed five small sessile submucosal tumors (SMTs) with a central depression or erosion in the duodenal bulb through descending part of the duodenum, which had been obscured beneath ulcers. Demonstrated in the regenerative mucosa by biopsy were clusters of small tumor cells, which, though considered the tips of neuroendocrine (NE) tumor (NET) in the deeper layer, were not large enough to be proven so by immuno staining with the markers in the serial sections, and diffuse hyperplasia of synaptophysin-, chromogranin A- and gastrin-positive NE cells in brunner glands (BGs), the preneoplastic lesion characteristic of MEN1-associated duodenal gastrinoma, supporting the diagnosis, which was firmly guaranteed by positively elevated glucagon-provoked plasma gastrin. Subtotal stomach-preserving pancreaticoduodenectomy established the final diagnosis of duodenal gastrinoma graded G1 associated with MEN1, which were shown to be tightly contained in the densely conglomerated hyperplastic BGs. Difficulty in endoscopically detecting the NET lies in the fact that, in addition to its smallness and deep localization, it might be buried under peptic ulcer at a certain stage and that an attempt to biopsy it is hampered by the densely conglomerated hyperplastic BGs in some cases.
 

Speaker
Biography:

Omesh Goyal is working as an Associate Professor in Gastroenterology and Hepatology in a tertiary care institute in northern India. He has done lot of research work on chronic hepatitis C and complications of cirrhosis. His other major interest includes functional bowel disorders and ano-rectal manometry. He is a part of the Indian working group on Chronic Constipation which will formulate guidelines for constipation in India under the leadership of Dr Uday Ghoshal. His research work in has been acclaimed at international level. He won the National Scholar Award at UEG in Sweden and Best paper award in APICON in Hyderabad, India. He is working as an editor of the Journal of Gastrointestinal Infections and is an active member of various academic bodies.

Abstract:

Statement of the Problem: In treatment-naïve patients with chronic hepatitis C(CHC) genotype 3(G-3) infection without cirrhosis, sofosbuvir plus daclatasvir daily for 12 weeks is the recommended therapy. In patients of CHC-G3 with cirrhosis, it is recommended to add daily ribavirin for 24 weeks along with the above combination, as data regarding the optimal duration of therapy in this subgroup is scarce. We aimed to study the SVR rates in CHC G3 patients with or without cirrhosis treated with sofosbuvir and daclatasvir. Methodology & Theoretical Orientation: Total 192 treatment naïve CHC-G3 patients treated with sofosbuvir and daclatasvir were enrolled. Of these, 112 did not have cirrhosis (group I), 42 had compensated cirrhosis (Child-Pugh A) (group II) and 32 patients had decompensated cirrhosis (Child Pugh B/C) (group III). Group I was treated with daily sofosbuvir(400 mg) and daily daclatasvir(60 mg) for 12 weeks, group II with daily sofosbuvir, daclatasvir and ribavirin(1000 or 1200 mg; weight based) for 12 weeks, and group III with sofosbuvir, daclatasvir and ribavirin for 24 weeks. HCV RNA was repeated at 12 weeks post-therapy for sustained virological response (SVR). Findings: Baseline characteristics in the three groups were similar (median age 48 years, 78% males). SVR rates in three groups are shown in figure. The SVR rate of group II was similar to group I (p< 0.0001). The SVR rate of group III was also similar to that of group II (p< 0.0001). No major adverse events were reported. On multivariate analysis, presence of decompensated cirrhosis was the only factor associated with relapse. Conclusion & Significance: This is the first study to show that patients of CHC G3 infection with compensated cirrhosis can achieve excellent SVR rate when treated with sofosbuvir, daclatasvir and ribavirin for 12 weeks. Patients with decompensated cirrhosis require triple therapy for 24 weeks.

 

Speaker
Biography:

Anna DePold Hohler is an Associate Professor of Neurology at Boston University School of Medicine.  Her research interests include “Autonomic research in Parkinson’s disease and postural tachycardia syndrome”. She has described the genetic association between POTS and Ehlers Danlos type III. Recently, she has explored gastrointestinal, sleep, and dermatologic disorders in this population. She has numerous publications and is an expert and frequent Reviewer.  She has dozens of publications and serves as an Associate Editor of Continuum, one of the premier neurology journals.  She is the recipient of numerous awards, including army achievement and commendation medals and two meritorious service medals for excellence in clinical and teaching skills while serving on active duty. She also received BUSM’s prestigious Stanley L. Robbins Award for Excellence in Teaching and several Neurology teaching awards, including two from the American Academy of Neurology (AAN). She co-chairs the AAN Quality Safety Subcommittee.

Abstract:

Postural Tachycardia Syndrome (POTS) is associated with a number of systemic effects including gastrointestinal (GI) dysfunction.  The most commonly reported GI symptoms are nausea, irregular bowel movements, abdominal pain, and constipation. Many POTS patients report GI symptoms more than once per week.  They often require a GI specialist. The POTS patients often have gastroparesis or delayed gastric emptying.  GI disturbances are frequent and prolonged in patients with POTS, impacting quality of life. Given the importance of the enteric nervous system to normal GI functioning, the same autonomic impairment leading to POTS may result in abnormal gut motility and ultimately subjective GI discomfort. Treatment of autonomic dysfunction in POTS and dietary changes may improve GI dysfunction associated with POTS. 

Speaker
Biography:

Hüseyin Sancar Bozkurt is currently working in Medical Park Tarsus Hospital, Turkey. He is the recipient of numerous awards for his expert research works in related fields. His research interests reflect in his wide range of publications in various national and international journals.
 

Abstract:

Background: Esophageal variceal bleeding is a life-threatening complication of portal hypertension with a six-week mortality rate of approximately 20%. The available data suggest that vasoactive drugs, combined with endoscopic therapy and antibiotics, are the best treatment strategy with endoscopic variceal ligation (EVL) being the endoscopic procedure of choice. Tissue adhesives, endoloops, endoscopic clipping and argon plasma coagulation (APC), have been used in the management of uncontrolled esophageal varices bleeding.
 
Methods: We reported two cases 77-year-old man with hepatitis C, Child-Pugh B cirrhosis who received EVL for esophageal variceal haemorrhage two years ago and 47-year old man with alcohol induced Child-Pugh C cirrhosis who received EVL for esophageal variceal haemorrhage one year ago included with uncontrolled esophageal varices bleeding.
 
Results: Argon plasma coagulation has been used as a recovery treatment for controlling of acute esophageal varices bleeding after unsuccessful endoscopic sclerotherapy and EVL (figure 1 and figure 2). The bleeding was controlled successfully in patients.
 
Conclusion: To our knowledge, this is the first documented case to report APC used in uncontrolled acute esophageal varices bleeding. Argon plasma coagulation can be endoscopic recovery treatment in uncontrolled esophageal varices bleeding.
 

Deog-Yong Lee

Korea National Institute of Health, South Korea

Title: Molecular epidemiological study of Norovirus related outbreak in Korea

Time : 15:40-16:00

Speaker
Biography:

Deog-Yong Lee has completed his Doctor of Veterinary Medicine (DVM) and Ph.D. at Seoul National University. He is a Staff Scientist and team leader of enteric virus team in Division of Enteric Disease, KCDC. He has published more than 100 papers in reputed journals.

Abstract:

Viral gastroenteritis was generally induced in a child less than 5 year old by type A rotavirus, enteric adenovirus, astrovirus, Sapovirus, except for norovirus. Th ese were designated as surveillance required pathogens legally and this was performed by KNIH (Korea National Institute of Health) as a name of EnterNet-Korea. In this point, we analyzed genotype of enteric virus in Korea to investigate evasion of host immune system. Norovirus is typical single-stranded (+) RNA virus and is divided into 5-genogroup. Genogroup I and II generally infect human. Norovirus GII.4 was main genotype in the world. GII.4 strain continuously mutated their genome and several GII.4 variants induced outbreaks, like as Sydney variant associated outbreak. In recent, GII.17 is emerging`genotype in south-east Asia and induced several outbreaks last winter seasons. Th is is the huge antigenic change of norovirus in south-east Asia and it may spread to other area. Recently, the genotype of other virus also changed and sometimes induced outbreak by minor genotypes in Korea. Genotypes provide important information about evading strategy of enteric virus from host immune system and this also provides tactical information to diagnose and prevent pathogens. Although we are not able to catch up the mutation rate of enteric virus, we must continuously follow up to decrease disease.

Speaker
Biography:

Nevena Ilic has completed her MhD in Endocrinology at Belgrade University Medical School in 2009 and Master’s degree in Thyroid Diseases in Italy, 2014. She completed Internal Medicine Specialisation at Military Medical Academy Hospital, Belgrade in 2002. From 2014, she works as Prime Endocrinologist at Euromedik General Hospital, Belgrade, where she organized endocrinology service and several symposiums in Belgrade and Rome, where she was a speaker. She spoke at several international congresses. She has published three papers in reputed journals as a first autor and many papers as the one of co-autors. She is a member of European and Italian Endocrinology Society. 

Abstract:

The gastrointestinal tract is an organ essential for the digestion and extraction of nutrients, but it’s also body’s largest endocrine organ. First discovered hormones at the beginning of the 20th century were gastrin, secretin and cholecystokinin. Now, we discuss about more than 30 different regulatory peptide hormones and more than 10 types of endocrine cells found in stomach, small and large intestines, such as incretins (glucose-dependent insulinotropic peptide and glucagon-like peptide-1), peptide YY, oxyntomodulin, ghrelin, obestatin and others. The role of gut hormones in energy homeostasis has been studied over the past 20 years. A great deal of researches in last years had shown the relationship between gastrointestinal hormones, obesity and type 2 diabetes, starting with incretin concept. Now, we have therapies based on gut hormones as targets, for patients with obesity, diabetes and non alcoholic fatty liver. Latest data present gut peptides as novel regulators of intestinal lipoprotein secretion and thus may have a great role in cardiovascular risk. Studies confirmed that gut hormones play a critical role in the regulation of metabolic, water and salt homeostasis and the development of hypertension and cardiovascular diseases. Recent investigations explained molecular mechanisms connecting gut hormones, insulin resistance and malignancies as well as inflammation processes in organism. Gut-brain axis and metabolism in polycystic ovary syndrome and it’s treatment has been a subject of polemics on recent endocrinology debates. Finally, there is a link between gut, adipose hormones and reproductive system and fertility in both sexes.

Speaker
Biography:

Purnama Andriana has completed his Digestive Surgeon education at Hasan Sadikin Hospital, Padjadjaran University, Indonesia. He attended many digestive surgery courses and fellowships, including Laparoscopic Colorectal Fellowship at Singapore General Hospital (2009), Minimal Invasive Surgery training at Academisch Medisch Centrum Amsterdam (2012) and, Endoscopy Laparoscopy training at Queen Mary Hospital, Hong Kong (2013). He has become Digestive and General Surgery Consultant Staff at Padjadjaran University, Indonesia.

Abstract:

Perforated gastric ulcer is still the most common indication for emergency gastric surgery associated with high morbidity and mortality. Outcome might be improved by performing laparoscopy. The aim of this study was to evaluate the outcome of laparoscopy at Hasan Sadikin Hospital. The outcome laparoscopy approach and the associated morbidity and mortality, operation time, conversion rate and hospital stay were assessed and compared with laparotomy. There were 30 patients (24 males, 6 females) with perforated gastric ulcer with mean age 72.14, non-malignant cause was documented during January 2015-Desember 2015. Patients with Boey’s score 0-1, ulcer diameter less than 2 cm at anterior site, underwent laparoscopic gastric perforation closure with omental patch and the rest were laparotomy. Observation from 15 patients underwent laparoscopy with no conversion, resulted in a better outcome from duration of operation 60-90 minutes (mean 79.57) than laparotomy 60-120 minutes (mean 85.73). Postoperative pain was found better outcome in laparoscopy VAS 3.93, laparotomy 6.27. Early diet was implemented in laparoscopy patients according to ERAS from POD one. Length of hospital stay in laparoscopy group was five days and 10-12 days (mean 10.5) in laparotomy. Incidence of surgical site infection was found in five patients, leakage from perforated site in six patients and mortality in six patients, all of them were found in laparotomy group. Laparoscopy closure of perforated gastric ulcer is a safe therapeutic method with strict selection of patient criteria. Based on low rates of morbidity and mortality, we should encourage laparoscopy implementation in gastric ulcer perforation case.

Ulrike Stein

Max Delbruck Center for Molecular Medicine, Germany

Title: Cancer metastasis biomarkers: Discover, develop, intervene

Time : 16:40-17:00

Speaker
Biography:

Ulrike Stein completed her Diploma degree at Martin-Luther University Halle, Germany and PhD at Humboldt University Berlin. For her Post-doctoral studies, she joined the laboratory of Dr. R H Shoemaker at National Cancer Institute/NIH Frederick as Feodor-Lynen-Fellow of Alexander von Humboldt foundation. She received her Habilitation at Charité Universitätsmedizin Berlin and appointed as Professor. She heads the research group of Translational Oncology of Solid Tumors at Experimental and Clinical Research Center, Charité Universitätsmedizin and Max-Delbrück-Center for Molecular Medicine in Berlin. Her research is focused on “Understanding and intervening in tumor progression and cancer metastasis formation”. She has published more than 130 papers in reputed journals. She is an Editorial Board Member and Reviewer of several journals. She received various national and international scientific awards.

Abstract:

Statement of the Problem: Metastasis is directly linked to colorectal cancer (CRC) patient survival and accounts for about 90% of patient deaths. It represents the most lethal event during the disease course and critically limits successful therapy.
 
Aim: Our translational concepts aim at the identification of key molecules such as S100A4 in tumor progression and metastasis for improved prognosis and therapy of solid cancers.
 
Methodology: We discovered key players of metastasis, their transcriptional targets, protein binding partners and signaling pathways thereof as new diagnostic, prognostic and predictive biomarkers for tumor progression and metastasis. Biomarker development was done in established and patient-derived 3D cultures, cell line-derived and patient-derived xenografts (PDX) and newly generated genetically engineered mouse models. We exploited this knowledge for improved disease prognosis and treatment response prediction in tissue and blood of cancer patients of several tumor entities. We established intervention strategies targeting biomarkers such as S100A4 for metastasis inhibition in mice.
 
Results: Small hairpin RNA (shRNA) acting on the biomarkers, on their transcriptional or post-translational targets decreased in vivo metastasis, also when applied systemically. In particular, small molecule transcriptional inhibitors were identified by high throughput screening, restricted biomarker-induced metastasis in mice. This repositioning of already FDA-approved drugs for the new indication of metastasis restriction paved the way for clinical trials.
 
Conclusion & Significance: We currently translate our findings on restricting S100A4-driven colorectal cancer metastasis into clinical practice. Novel therapeutic approaches targeting S100A4 are currently tested in phase II clinical trials to treat patients with metastatic disease. Our assay for detecting and quantifying circulating biomarker transcripts in patient blood is used to monitor treatment success.
 

Dmitriy Shamrai

National Cancer Institute, Ukraine

Title: Complications in Esophageal Surgery

Time : 17:00-17:20

Speaker
Biography:

Kondratskyy Y is Head of Esophageal and Gastric Tumors department at National Cancer Institute, Ukraine. He is the recipient of numerous awards for his expert research works in related fields. His research interests reflect in his wide range of publications in various national and international journals.

Abstract:

Background & Aim: Despite esophagectomy is common surgical procedure, high complication rate, their early detection and management remains challenging problem. The aim of the study is to find out complications in esophageal surgery.
 
Methods: We reviewed medical documentation of patients who underwent esophagectomy at National Cancer Institute (Kiev, Ukraine) between January 2010 and December 2016. Esophagectomies were done in Lewis, McKeown and transhiatal manner. We performed three field dissections in patients with upper third tumors of the esophagus with clinical lymph node metastases in the superior mediastinum; the others underwent two field dissection. All esophagectomies were done by one team of surgeons. We analyzed complications according to Clavien-Dindo classification and role of early endoscopy in prediction of anastomotic problems.
 
Results: 300 patients with esophageal cancer were operated: 285 Lewis, 12 McKeown and three transhiatal esophagectomies. Postoperative complication rate was 24.3% (73 cases), perioperative mortality rate–3% (nine patients). Surgical complications grade I-II took place in 23 patients, grade III–27 cases; grade IV 23 cases (according to Clavien-Dindo classification). The most frequent complications were pneumonia (n=14) and pleural effusion (n=9). Recurrent laryngeal nerve palsy developed only in one patient. We divided life-threatening complications (grade IV) into surgical (anastomotic leak n=7, empyema n=4, mediastinitis n=3) and non-surgical groups (pulmonary embolism n=7, myocardial infarction n=3). All symptomatic anastomotic leaks (n=7) were operated and anastomotic structures (n=4) were stented. Early endoscopy (within 1 week after operation) was done in 156 patients. It helped to predict anastomotic problems in six cases (true positive results).
 
Conclusion: Despite non-surgical complications led to death more frequently, they were always accompanied by surgical complications. To minimize anastomotic leaks rate, surgical technique and surgeon’s experience (more than 40 esophagectomies every year) is crucial. Early endoscopy can predict anastomotic problems and would be investigated further.
 

Baris Cankaya

Marmara University, Turkey

Title: Best fluid management for bariatric surgery: Restrictive or Liberal

Time : 17:20-17:40

Speaker
Biography:

Baris Cankaya completed his Graduation at Ankara University Medical Faculty in 2000. He has been working as Anaesthesiology Specialist at Marmara University Training Hospital. He has attended academic meetings, nationally and internationally. His academic interest includes “Microcirculation, fluid therapy, resuscitation, patient safety and perioperative analgesia”. Some of his certificates are EPLS provider Berlin 2015, NLS provider Athens 2015 and MECOR Level I October 2014. He attended international workshops like ECMO workshop 2015, Leicester and Airway workshop, ICISA 2014, and Tel Aviv.

Abstract:

Bariatric and metabolic surgery procedures are choice of treatment with an increasing number worldwide. Fluid management is a topic of debate for many years. Restrictive and liberal fluid protocols are under research of clinical trials. Which fluid regime should be the best for bariatric procedures? A low fluid administration may lead to decreased circulating volume, redistribution of plasma, decreased urine output. On the other hand, fluid overload may cause complications as edema, raised central venous pressure, deformation of glycocalyx. There is intestinal edema can lead to impaired tolerance for enteral nutrition. Randomized 48 American Society of Anesthesiologists (ASA) grade 1–3 patients for cholecystectomy operation have been compared with liberal (40 ml/kg) and restricted (15 ml/kg) fluid regime. It has been reported that those receiving liberal therapy had fewer postoperative complications. For a long time, it has been accepted that liberal perioperative intravenous fluid administration was better. There are now more evidence that restricted regimen would be more suitable for fast track colon surgery. Major abdominal surgery has risks, economic results and postoperative complications. Latest RELIEF trial (restrictive versus liberal fluid therapy in major abdominal surgery) has found out preliminary supportive evidence for restrictive regimen for major abdominal surgery. As a conclusion, the perioperative fluid regimen should be individualized. Fluid regimen plays an important role on hospital length of stay for patients undergoing laparoscopic bariatric surgery.
 

  • Poster Presentations
Location: Olimpica 2
Speaker

Chair

David H. Van Thiel

Advanced Liver and Gastrointestinal Disease Center, USA

Speaker
Biography:

Esam Elshimi is working at Menoufia University, Egypt. He is the recipient of numerous awards for his research works in related fields. His research interests reflect in his wide range of publications in various national and international journals.

Abstract:

Introduction & Aim: Heavy iron overload is toxic to virtually all cells and tissues. There is growing evidence that only modest amounts of iron in the liver may serve as a co-morbid factor to increase the severity and/or rate of progression of liver disease. Aim of this study is to explore the role of iron, HFE mutations, and polymorphisms of the TfR1 gene in the progression of chronic hepatitis C infection and possible therapeutic implications of iron overload on interferon therapy of patients with chronic hepatitis C.
 
Methods: From 3rd October 2012 to 6th January 2016, we studied 300 consecutive patients with chronic hepatitis C, correlating clinical, laboratory, histopathological, and genetic data. Frequencies of genetic variations were compared with healthy controls.
 
Results: HFE mutations were more common in patients than controls (25% vs. 11.7%, P=0.00006), and the C282Y mutation were more common in patients than controls (38.0% vs. 48.0%, P=0.02). Patients carrying C282Y had higher mean hepatic iron concentrations (P=0.02). Hepatic fibrosis was correlated with hepatic iron concentration (P=0.03). HFE and TfR1 polymorphisms bore detectable relation to disease severity and to response to interferon therapy.
 
Conclusions: Hepatic iron and HFE andTfR1mutations are co-morbid factors that increase progression of chronic hepatitis C and decrease the response to interferon therapy.
 

Speaker
Biography:

Esam Elshimi is working at Menoufia University, Egypt. He is the recipient of numerous awards for his research works in related fields. His research interests reflect in his wide range of publications in various national and international journals.

Abstract:

Background: Matrix metalloproteinase-12(MMP-12) is involved in tumor invasiveness and metastasis and significantly over-expressed in tissues of HCC.
 
Aim: Aim of this study was to investigate the diagnostic and prognostic value of serum mRNA MMP-12 over expression in human HCC on top of HCV related cirrhosis.
 
Subjects & Methods: From January 2016 to June 2016, 50 patients with chronic HCV related cirrhosis and HCC, 50 patients with HCV related cirrhosis compared to 50 healthy persons as a control group. They were selected from National Liver Disease–Menoufia University; all patients were subjected to tri-phasic CT abdomen when indicated, liver profile, AFP and molecular characterization of metalloproteinase-12 expression by molecular biology techniques.
 
Results: There were statistically significant differences between HCC and cirrhotic patients versus control group regarding (CBC parameters and liver profile), (p-value<0.01), while there were no differences between all groups regarding creatinine (p-value>0.05). There was a statistically significant difference between HCC patients and other groups regarding mRNA-MMP12 (p-value<0.01), sensitivity 72.0%, specificity 60.0%, AUC0.68, accuracy 70.9%, P value<0.01, mRNA-MMP12 and/or AFP had sensitivity of 84.0%, specificity 60.0%, PPV of 51.2, NPP 88.2%. Accuracy of mRNA-MMP12 and/or AFP=68.0%. The sensitivity of mRNA-MMP12 and AFP= 58.0 %, specificity 78.0%, PPV 56.9%, NPV 78.8% and accuracy 71.3%.
 
Conclusion: mRNA-MMP12 is a good sensitive, bad specific but accurate in diagnosing HCC. Adding serum mRNA-MMP12 to AFP improves early diagnosis and hence, better prognosis.
 

Speaker
Biography:

Hüseyin Sancar Bozkurt has completed his PhD at the age of 24 years from Trakya University and  Ä°nternal Medicine at the age of 30 from Çukurova University. He has completed gastroenterology education at the age of 33 years from Adana BaÅŸkent University. He has published multiple national and international papers.

Abstract:

Background: Clostridium difficile is a major cause of intestinal infection and diarrhoea in individuals following antibiotic treatment. Disease associated with C. difficile infection (CDI) ranges from mild diarrhoea to pseudomembranous colitis (PMC). Severe CDI unresponsive to intravenous (IV) metronidazole therapy requires more aggressive medical management and possible surgical intervention. In the case of ileus, intracolonic and oral vancomycin presented a promising alternative method for administering the antibiotic.
 
Methods: We reported a five year old boy had non bloody diarrhea with un-responding metronidazole treatment for 10 days. The stool CDI cytotoxin assay was negative. The patient had no antibiotic exposure in the six weeks prior to diarrhoea. Abdominal pain, ileus, fever, leukocytosis were occurred (figure 1). Decompressive flexible sigmoidoscopy revealed inflamed mucosa and yellow plaque like lesions in sigmoid and descending colon (figure 2). Stool cultures and analysis for Rotavirus, Staphylococcus, Shigella, Salmonella and Candida were negative.
 
Results: Ä°ntraluminal vancomycin (1 gr in 250 ml serum physiologic) was performed during flexible sigmoidoscopy. Oral vancomycin was started (40 mg/kg) four times a day. The patient’s condition improved after treatment and three days later soft diet started (figure-3).
 
Conclusion: Pediatric CDI cases found 87% reported only diarrhea, 9% had severe CDI and 4% had severe CDI with complications (toxic megacolon, ileus, intestinal perforation). In the case of ileus, intracolonic and oral vancomycin presented a promising alternative method for administering the antibiotic in clinical suspect of CDI associated PMC. To our knowledge, this is the first documented case to report successful intracolonic and oral vancomycin treatment used in a child patient. 
 

Speaker
Biography:

Statement of the Problem: Functional constipation (FC) and constipation-predominant irritable bowel syndrome (IBS-C) are a part of functional bowel disorders, and have a significant personal, healthcare, and social impact. Evaluation by anorectal manometry is essential in these cases for targeted treatment. Data on the anorectal manometric abnormalities in these patients is scarce. We aimed to study the anorectal manometric abnormalities in patients with functional constipation and constipation-predominant IBS in northern India. Methodology & Theoretical Orientation: A total of 114 consecutive patients with the history of chronic constipation who underwent anorectal manometry from January 2013 to December 2016 in a tertiary care institute were enrolled. Standard laboratory tests and colonoscopy were normal. Twenty-six healthy volunteers served as controls. Finding: The mean age was 46.7 years, 75.4% were males, and the median duration of constipation was 60 months. Sixty-two patients satisfied ROME IV criteria for functional constipation (FC) and 52 had Irritable bowel syndrome- constipation predominant (IBS-C). A comparison of the anorectal motor and sensory manometry parameters along with healthy controls is shown in the table. The resting anal pressure and the squeeze pressure were similar in all subgroups. Dyssynergic pattern of defecation was seen in significantly more patients in the FC group (p<0.001). The first sensation threshold was significantly higher among FC patients. The thresholds for the desire to defecate and maximum tolerable volume were significantly higher among FC and IBS-C patients compared to controls. Conclusions and significance: Dyssynergic pattern of defecation is seen in significantly more patients with FC compared to IBS-C. Patients with FC have marked the elevation of all sensory thresholds, while IBS-C patients have similar first sensation threshold with the elevated threshold for urge and maximum tolerance.

Abstract:

Omesh Goyal is working as an Associate Professor in Gastroenterology and Hepatology in a tertiary care institute in northern India. He has done a lot of research work on chronic hepatitis C and complications of cirrhosis. His other major interest includes functional bowel disorders and ano-rectal manometry. He is a part of the Indian working group on Chronic Constipation which will formulate guidelines for constipation in India under the leadership of Dr. Uday Ghoshal. His research work in has been acclaimed at international level. He won the National Scholar Award at UEG in Sweden and Best paper award in APICON in Hyderabad, India. He is working as an editor of the Journal of Gastrointestinal Infections and is an active member of various academic bodies.

Shuaib Meghji

University Hospital Southampton, United Kingdom

Title: Comparison of the performance of LTBI screening to the BTS standards
Speaker
Biography:

Shuaib Meghji is currently studying at University Hospital Southampton, UK.

Abstract:

Background: Patients with severe Inflammatory Bowel Disease (IBD) are prescribed anti-TNF-α agents, if clinical need necessitates, whose immunosuppressive action can potentially reactivate latent tuberculosis infections (LTBI). Meticulous pre anti-TNF-α LTBI screening and management in accordance with the British Thoracic Society’s (BTS) Guidelines is imperative for patient safety and public health. 
 
Objective: A retrospective clinical audit was performed to evaluate the performance of University Hospital Southampton’s Gastroenterology department in screening for LTBI in patients with IBD. The performance of LTBI screening was compared to the BTS standards. 
 
Method: The audit population was obtained using the gastroenterology department’s biologics database. Inclusion criteria included patients who started their first anti-TNF-α agent between 01/01/2006 to 04/11/2016. Exclusion criteria included deceased patients and patients screened by alternative departments/trusts. Extent of LTBI screening was assessed using hospital record systems: EDocs, EQuest, ECamis and Spectra PACS. If evidence of screening was not located, then this was considered as a failure to meet standard. Following statistical analysis, comparisons were made with BTS standards. 
 
Results: Of the 471 patients audited, 51.2% were females and 48.8% males. 75.2% were CD patients and 24.8% were UC patients. 231 patients’ (49%) LTBI screening was insufficient. 157 patients (33.3%) lacked an adequate TB history and 94 patients (20%) failed to have a chest radiograph (CXR) within three months of therapy commencement. Additionally, 85 patients (18.3%) failed to have an IGRA performed. 15 patients (3.2%) were diagnosed with LTBI, while one case of TB reactivation occurred once immunosuppressive therapy had commenced. 
 
Conclusion: The completeness of LTBI screening in the audited group was suboptimal with deficits in TB history performance, CXR, TST and IGRAs. One case of active miliary TB occurred as a result of inadequate screening. In light of this, recommendations to address deficits and ultimately improve screening were proposed.